Block-staining tissues with potassium ferrocyanide-reduced osmium tetroxide and lead aspartate for electron microscopic radioautography.
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چکیده
منابع مشابه
Fixation of Neural Tissues for Electron Microscopy by Perfusion with Solutions of Osmium Tetroxide
This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic le...
متن کاملScanning electron microscopic observation of Bruch's membrane with the osmium tetroxide treatment.
Scanning electron microscopic observation of Bruch's membrane was performed after removal of retinal pigment epithelium (RPE) with the osmium tetroxide treatment. Eight human eyes from subjects at various ages (from newborn to 77 years old) were examined in order to investigate aging changes in Bruch's membrane. The collagen fibres of the inner collagenous zone in young eyes formed a tightly in...
متن کاملContrast staining with reduced osmium complexes.
Recently I read a Technical Note in the Journal of Histocbemistv and Cytocbemz'try (1) entitled: " Improved method for post-embedding cyto-chemistry using reduced osmium and LR White Resin, " by Hideaki Tamaki and Shohei Yamashina. On the one hand, I was glad to see the revival of the use of ferrocyanide as an additive to Os04 post-fixation in LR White resin procedures. On the other hand, I was...
متن کاملNuclear Precipitates in Pyroantimonate-osmium Tetroxide-fixed Tissues
Recent biochemical studies indicate a significant role for sodium ions in nuclear metabolism. Protein and nucleic acid syntheses in the nucleus depend upon sodium, apparently through a stimulating effect of this cation on the transport of amino acid, purine, and pyrimidine into the nucleus (1). The nuclei of avian erythrocytes and calf thymocytes appear to contain higher sodium concentrations t...
متن کاملPerfusion fixation with glutaraldehyde and post-fixation with osmium tetroxide for electron microscopy.
The conductivity of cerebral cortex drops during perfusion with glutaraldehyde in 5 min to about 60% of the original value, to remain unchanged during the subsequent 10-15 min of perfusion. Circulatory arrest causes a similar drop in the tissue conductivity. Perfusion of asphyxiated tissue with glutaraldehyde does not produce additional major changes in the conductivity. Perfusion of the cortex...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1984
ISSN: 0022-1554,1551-5044
DOI: 10.1177/32.5.6201530